Results
| PMID | 12372466 |
| Gene Name | CCL2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 10 |
| Population details | 10 (5 women undergoing surgery for pelvic pain, infertility, or endometriosis; 5 women without endometriosis who were undergoing tubal ligation were the controls ) |
| Sex | Female |
| Infertility type | Female infertility |
| Other associated phenotypes |
Endometriosis, Infertility |
|
Fertil Steril. 2002 Oct;78(4):843-8. Rong, Rong| Ramachandran, Sumathi| Santanam, Nalini| Murphy, Ana A| Parthasarathy, Sampath Emory Center for Advanced Research on Women's Health, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA. OBJECTIVE: To elucidate the effect of oxidized low-density lipoprotein (LDL) and peritoneal fluid of women with endometriosis on monocyte chemotactic protein-1 (MCP-1) production by peritoneal mesothelial cells and endometrial cells. DESIGN: In vitro study. SETTING: University medical center. PATIENT(S): Five women undergoing surgery for pelvic pain, infertility, or endometriosis; five women without endometriosis who were undergoing tubal ligation were the controls. INTERVENTION(S): Mesothelial cells and endometrial cells in culture were treated with oxidized LDL and peritoneal fluid from control and endometriosis patients, then MCP-1 levels were measured. MAIN OUTCOME MEASURE(S): ELISA was used to measure MCP-1 in the culture supernatants exposed to oxidized LDL and peritoneal fluid from control and endometriosis patients. Cellular MCP-1 messenger RNA expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) assay. RESULT(S): Treatment with oxidized LDL caused an increase in accumulation of immunoreactive MCP-1 in the medium of cultured mesothelial and endometrial cells (primary endometrial stromal cells and endometrial cell line EM42). The mesothelial cells secreted more MCP-1 than did endometrial cells under the culture condition. The EM42 cells cultured in the presence of peritoneal fluid from endometriosis patients secreted more MCP-1 than those cultured with peritoneal fluid from normal women. However, no differences were found in MCP-1 levels in the supernatant of endometrial stromal cells cultured with peritoneal fluid. CONCLUSION(S): This is the first report of MCP-1 expression in mesothelial cells induced by oxidized LDL, and provides direct evidence of inflammatory action of peritoneal fluid of women with endometriosis. Mesh Terms: Ascitic Fluid/*physiopathology| Cells, Cultured| Chemokine CCL2/analysis/*biosynthesis| Culture Media, Conditioned/chemistry| Endometriosis/*metabolism/pathology| Endometrium/drug effects/*metabolism/pathology| Enzyme-Linked Immunosorbent Assay| Ep |
|